Jump to Main Content
Efficient Separation and Analysis of Triacylglycerols: Quantitation of Î²-Palmitate (OPO) in Oils and Infant Formulas
- Vyssotski, Mikhail, Bloor, Stephen J., Lagutin, Kirill, Wong, Herbert, Williams, D. Bradley G.
- Journal of agricultural and food chemistry 2015 v.63 no.26 pp. 5985-5992
- glycerol, infant formulas, isomers, light scattering, oils, quantitative analysis, reversed-phase high performance liquid chromatography, triacylglycerols
- A high-efficiency, convenient, and reliable method for the separation of structurally similar triacylglycerols is detailed and applied in the quantitative analysis of 1,3-dioleoyl-2-palmitoylglycerol (OPO) in infant formulas and OPO oils. OPO is an important lipid component in âhumanizedâ infant formula. A fast preparative isolation of an OPO-containing fraction from the crude complex mixture, by nonaqueous reversed phase HPLC, followed by Ag+-HPLC with detection at 205 nm allowed fine separation and detection of the desired fraction. OPO was quantitated independently of its regioisomer 1,2-dioleoyl-3-palmitoylglycerol (OOP) and isomers of stearoyl-linoleoyl-palmitoyl glycerol that might be present in infant formulas. For samples with low OPO content, an evaporative light-scattering detector (ELSD) was more preferable than UV detection, with a calculated LOD of 0.1 Î¼g of OPO injected and LOQ of 0.3 Î¼g. The method, which showed high reproducibility (RSD < 5%), was suitable for both high OPO content oils and low OPO products such as unenriched infant formula. A number of possible interference issues were considered and dealt with.