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Biocontrol Activity of Bacillus subtilis Isolated from Agaricus bisporus Mushroom Compost Against Pathogenic Fungi

Author:
Liu, Can, Sheng, Jiping, Chen, Lin, Zheng, Yanyan, Lee, David Yue Wei, Yang, Yang, Xu, Mingshuang, Shen, Lin
Source:
Journal of agricultural and food chemistry 2015 v.63 no.26 pp. 6009-6018
ISSN:
0021-8561
Subject:
Agaricus bisporus, Bacillus subtilis, Neurospora sitophila, antifungal agents, antifungal properties, biological control, breads, desorption, electrospray ionization mass spectrometry, ionization, lipopeptides, molecular weight, mushroom compost, mushrooms, mycelium, pH, plant pathogenic fungi, polyacrylamide gel electrophoresis, proteinases, spore germination, temperature
Abstract:
Bacillus subtilis strain B154, isolated from Agaricus bisporus mushroom compost infected by red bread mold, exhibited antagonistic activities against Neurospora sitophila. Antifungal activity against phytopathogenic fungi was also observed. The maximum antifungal activity was reached during the stationary phase. This antifungal activity was stable over a wide pH and temperature range and was not affected by proteases. Assay of antifungal activity in vitro indicated that a purified antifungal substance could strongly inhibit mycelia growth and spore germination of N. sitophila. In addition, treatment with strain B154 in A. bisporus mushroom compost infected with N. sitophila significantly increased the yield of bisporus mushrooms. Ultraviolet scan spectroscopy, tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis, matrix-associated laser desorption ionization time-of-flight mass spectrometry, and electrospray ionization tandem mass spectrometry analyses revealed a molecular weight consistent with 1498.7633 Da. The antifungal compound might belong to a new type of lipopeptide fengycin.
Agid:
4304961