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Dietary Plasma Proteins Modulate the Adaptive Immune Response in Mice with Acute Lung Inflammation

Author:
Maijó, Mònica, Miró, Lluïsa, Polo, Javier, Campbell, Joy, Russell, Louis, Crenshaw, Joe, Weaver, Eric, Moretó, Miquel, Pérez-Bosque, Anna
Source:
Journal of nutrition 2012 v.142 no.2 pp. 264-270
ISSN:
0022-3166
Subject:
anti-inflammatory activity, dietary protein, interleukin-12, lungs, blood proteins, protein supplements, interferon-gamma, interleukin-5, lymphocytes, pneumonia, weaning, mice, transforming growth factor beta, intestinal mucosa, immune response, interleukin-10, animal disease models, Escherichia coli
Abstract:
We examined the effects of oral plasma protein supplements on the pulmonary adaptive immune response in mice challenged with intranasal LPS. C57BL/6 mice were fed a control diet or a diet supplemented with plasma proteins [spray-dried plasma (SDP) 80 g/kg] or with an Ig concentrate [(IC) 20 g/kg] from postnatal d 19 (weaning) until d 34. Mice were challenged with PBS or LPS from Escherichia coli at d 33 and killed 24 h later for leukocyte analyses or at d 34 and killed 6 h later for cytokine determination. LPS induced the activation of T helper (Th) lymphocytes in lung and blood and this response was reduced by SDP and IC (P < 0.05). In both tissues, LPS increased the Th1 and Th2 subpopulations and this effect was inhibited by the two plasma protein supplements (P < 0.05). The LPS challenge increased the expression of all the cytokines studied (P < 0.01). SDP and IC reduced the expression of IFNγ, IL-5, IL-12p40, IL-12p70, IL-13, and IL-17 in both tissues, whereas they increased the percentage of regulatory Th lymphocytes in lung, even in PBS-treated mice (P < 0.05). LPS reduced the concentration of mature TGFβ1 (P < 0.05) in the lung but did not modify the expression of IL-10. Mice exposed to LPS and supplemented with SDP or IC showed an increased expression of the anti-inflammatory cytokine IL-10 (P < 0.05). Moreover, the two supplements increased the concentration of IL-10 in intestinal mucosa (P < 0.05). Our results show that plasma supplementation reduces the immune response that characterizes the acute lung inflammation syndrome.
Agid:
432703