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Isolation and identification of cytotoxic compounds from the wood of Pinus resinosa
- Simard, François, Legault, Jean, Lavoie, Serge, Mshvildadze, Vakhtang, Pichette, André
- Phytotherapy research 2008 v.22 no.7 pp. 919-922
- Pinus resinosa, adenocarcinoma, cytotoxicity, ethers, humans, lungs, mass spectrometry, methanol, nuclear magnetic resonance spectroscopy, wood, wood extractives
- Methanol extracts of wood from Pinus resinosa were found to be selectively cytotoxic against human lung carcinoma cells, A549 (IC₅₀ 41 ± 6 µg/mL), human colorectal adenocarcinoma cells, DLD‐1 (IC₅₀ 47 ± 4 µg/mL) in comparison with healthy cells, WS1 (IC₅₀ 130 ± 11 µg/mL). Five known compounds were isolated and identified by ¹H, ¹³C NMR spectroscopy and HR‐ESI‐MS mass spectrometry as, pinosylvin monomethyl ether (1), pinosylvin (2), pinosylvin dimethyl ether (3), pinobanksin (4) and (‐)‐norachelogenin (5). Compound 4 was isolated for the first time in P. resinosa. The cytotoxicity of compounds 1–5 was evaluated against A549, DLD‐1 and WS1. Compound 1 exhibited the strongest cytotoxicity against both tumor cell lines and the healthy cell line with an IC₅₀ of 25 ± 4 µm for A549, 20 ± 1 µm for DLD‐1 and 34 ± 3 µm for WS1. Copyright © 2008 John Wiley & Sons, Ltd.