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Characterization of 20 polymorphic microsatellites for Blunt snout bream (Megalobrama amblycephala) from EST sequences

Author:
Luo, Wei, Deng, Wei, Yi, Shaokui, Wang, Weimin, Gao, Zexia
Source:
Conservation genetics resources 2013 v.5 no.2 pp. 499-501
ISSN:
1877-7252
Subject:
Megalobrama amblycephala, alleles, chromosome mapping, databases, economics, expressed sequence tags, fish, genetic variation, heterozygosity, loci, marker-assisted selection, microsatellite repeats, population genetics, China
Abstract:
Blunt snout bream (Megalobrama amblycephala) is an important economic fish species in China, yet little is known about its genetic diversity. We describe the isolation and characterization of M. amblycephala from expressed sequence tags (ESTs) database. Forty-five loci were randomly selected for primer development, of these, 20 (44.4 %) showed polymorphic. Number of alleles ranged from 2 to 9 with an average of 4.10 per locus, expected heterozygosity and observed heterozygosity ranged from 0.031 to 1 and from 0.031 to 0.772, respectively. Seven microsatellite loci exhibited the deviation from from Hardy–Weinberg Equilibrium significantly (P < 0.05). These newly developed markers would be valuable for population genetics, genetic mapping and marker assisted selection of M. amblycephala.
Agid:
437452