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Kinetic method for acetylsalicylic acid determination based on its inhibitory effect upon the catalytic decomposition of H2O2

Author:
Mureşanu, Claudia, Copolovici, Lucian
Source:
Analytical and bioanalytical chemistry 2004 v.378 no.7 pp. 1868-1872
ISSN:
1618-2642
Subject:
aspirin, catalase, detection limit, hydrogen peroxide, oxygen, quantitative analysis
Abstract:
The catalytic reaction of catalase was investigated, by means of a Clark oxygen sensor, in the presence of various concentrations of acetylsalicylic acid. Michaelis-Menten kinetic parameters were determined from Lineweaver-Burk plots, obtained in the absence and in the presence of the inhibitor. The inhibition pattern, suggested by the Lineweave-Burk plots, corresponds to a fully mixed inhibition mechanism. A kinetic method, based on the indicator reaction: [Formula: see text], was developed for the quantitative determination of acetylsalicylic acid. Calibration graphs of the reciprocal value of first-order rate constant versus acetylsalicylic concentration covered the concentration range (2.99–19.98)×10⁻⁴ mol/L, while the detection limit was 4.12×10⁻⁴ mol/L acetylsalicylic acid with a standard deviation of 2.1×10⁻⁵ mol/L.
Agid:
4380766