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Micro- and nanofluidics for DNA analysis
- Tegenfeldt, Jonas O., Prinz, Christelle, Cao, Han, Huang, Richard L., Austin, Robert H., Chou, Stephen Y., Cox, Edward C., Sturm, James C.
- Analytical and bioanalytical chemistry 2004 v.378 no.7 pp. 1678-1692
- DNA, dielectrophoresis, epigenetics, gels
- Miniaturization to the micrometer and nanometer scale opens up the possibility to probe biology on a length scale where fundamental biological processes take place, such as the epigenetic and genetic control of single cells. To study single cells the necessary devices need to be integrated on a single chip; and, to access the relevant length scales, the devices need to be designed with feature sizes of a few nanometers up to several micrometers. We will give a few examples from the literature and from our own research in the field of miniaturized chip-based devices for DNA analysis, including dielectrophoresis for purification of DNA, artificial gel structures for rapid DNA separation, and nanofluidic channels for direct visualization of single DNA molecules.