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Determination of ochratoxin A in pig tissues by liquid–liquid extraction and clean-up and high-performance liquid chromatography

Monaci, L., Tantillo, G., Palmisano, F.
Analytical and bioanalytical chemistry 2004 v.378 no.7 pp. 1777-1782
detection limit, high performance liquid chromatography, kidneys, liquid-liquid extraction, liver, muscles, ochratoxin A, ochratoxin B, solid phase extraction, solvents, surveys, swine, Italy
A fast, simple, and sensitive HPLC–FD method is described for determination of ochratoxin A (OTA) in pig kidney and muscle; a small mass (<2.5 g) of sample and a relatively small volume (<15 mL) of a non-halogenated extraction solvent are required. Ochratoxin B, systematically absent from all the samples investigated, was used as internal standard. Liquid–liquid partition was used for sample clean-up. Recoveries at the 1 ng g⁻¹ level were 86±15% and 74±8% for kidney and muscle, respectively, and detection limits were 0.14 and 0.15 ng g⁻¹. Clean-up by solid-phase extraction (SPE) is required for pig liver. A survey of the OTA content of tissues of pigs slaughtered in southern Italy revealed that 52 out of 54 analysed samples were contaminated; the OTA concentration in kidney ranged between 0.26 and 3.05 ng g⁻¹. The effect of measurement precision on compliance with legal limits is also discussed.