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Development of a novel enzyme reactor and application as a chemiluminescence flow-through biosensor

Yang, Chunyan, Zhang, Zhujun, Shi, Zuolong, Xue, Pan, Chang, Pingping, Yan, Ruifang
Analytical and bioanalytical chemistry 2010 v.397 no.7 pp. 2997-3003
adsorption, biosensors, calcium alginate, catalytic activity, chemiluminescence, enzyme activity, glucose oxidase, immobilized enzymes, polymers, silica
A novel enzyme reactor was prepared using calcium alginate fiber (CAF) and amine-modified nanosized mesoporous silica (AMNMS) as a support. Combination of the adsorption of the enzyme on AMNMS with the cage effect of the polymer greatly increases the catalytic activity and the stability of the immobilized enzyme. It was shown that the lifetime, stability, and catalytic activity of the enzyme reactor were greatly improved by incorporating AMNMS into CAF to efficiently encapsulate the enzyme. Glucose oxidase was chosen as a model enzyme to explore the possibility of using CAF–AMNMS as a matrix for enzyme immobilization in the design of a chemiluminescence (CL) flow-through biosensor. The sensitivity of the flow-through biosensor combined with a novel luminol-diperiodatonickelate CL system was higher than for other reported CL biosensors. The proposed biosensor exhibits short response time, easy operation, long lifetime, high catalytic activity, high sensitivity, and simple assembly.