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Kinetics and Mechanism of Fenpropathrin Biodegradation by a Newly Isolated Pseudomonas aeruginosa sp. Strain JQ-41

Song, Haihai, Zhou, Zhiren, Liu, Yuanxiu, Deng, Si, Xu, Heng
Current microbiology 2015 v.71 no.3 pp. 326-332
Pseudomonas aeruginosa, bifenthrin, biodegradation, bioremediation, carbon, cyhalothrin, cypermethrin, deltamethrin, energy, fenpropathrin, gas chromatography-mass spectrometry, genes, hydrophobicity, metabolites, models, orchards, pH, pesticide application, pyrethrins, ribosomal DNA, soil bacteria
A soil bacterium designated strain JQ-41, capable of growth on fenpropathrin as the sole carbon source and energy source, was isolated from a long-term pyrethroid insecticide-treated orchard. Based on the morphology, physio-biochemical characteristics, and 16S rDNA gene analysis, as well as the G+C content of the genomic DNA, the strain JQ-41 was identified as Pseudomonas aeruginosa. Up to 92.3 % of 50 mg l⁻¹ fenpropathrin was degraded by P. aeruginosa strain at 30 °C and pH 7 within 7 days. The kinetic parameters q ₘₐₓ, K ₛ, and K ᵢ were established to be 1.14 day⁻¹, 38.41 mg l⁻¹, and 137.67 mg l⁻¹, respectively, and the critical inhibitor concentration was determined to be 72.72 mg l⁻¹. Cell surface hydrophobicity of P. aeruginosa strain was enhanced during growth on fenpropathrin. Three metabolites from fenpropathrin degradation were identified by gas chromatography mass spectrometry, and then a possible degradation pathway was proposed. In addition, this isolate was also able to degrade a wide range of synthetic pyrethroid insecticides including cypermethrin, deltamethrin, bifenthrin, and cyhalothrin with the degradation process following the first-order kinetic model. Taken together, our results provide insights into the kinetics and mechanism of fenpropathrin degradation by P. aeruginosa strain and also highlight its promising potential in bioremediation of pyrethroid-contaminated environment.