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Denaturing gradient gel electrophoresis (DGGE) as a rapid method for assessing gastrointestinal tract microflora responses in laying hens fed similar zinc molt induction diets

Ricke, S.C., Hume, M.E., Park, S.Y., Moore, R.W., Birkhold, S.G., Kubena, L.F., Nisbet, D.J.
Journal of rapid methods and automation in microbiology 2004 v.12 no.1 pp. 69
laying hens, gel electrophoresis, gastrointestinal system, intestinal microorganisms, zinc, molting, dietary supplements, animal nutrition, cecum, denaturing gradient gel electrophoresis
Induced molting through feed withdrawal can change the microenvironment of crop and ceca sufficiently to allow them to be the sites of Salmonella colonization in the chicken intestine. This study compares the denaturing gradient gel electrophoresis (DGGE) profiles of microbial crop and cecal communities among molted hens fed similar zinc acetate or zinc propionate amended molt diets to hens either undergoing feed withdrawal or hens full fed and not molted. Dendrograms of DGGE amplicon patterns indicated over 85% similarity of cecal communities between zinc acetate fed hens and zinc propionate fed hens and over 60% similarity of crop communities between zinc acetate fed hens and zinc propionate fed hens. Rapid comparison of complex gastrointestinal microflora profiles in laying hens fed similar diets is possible using DGGE.