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Modeling of the pyruvate production with Escherichia coli in a fed-batch bioreactor

Zelić, B., Vasić-Rački, Đ., Wandrey, C., Takors, R.
Bioprocess and biosystems engineering 2004 v.26 no.4 pp. 249-258
Escherichia coli, acetates, acetyl coenzyme A, batch fermentation, biomass, bioreactors, carbon, cell growth, feeding methods, glucose, growth retardation, model validation, models, pyruvic acid
A family of 10 competing, unstructured models has been developed to model cell growth, substrate consumption, and product formation of the pyruvate producing strain Escherichia coli YYC202 ldhA::Kan strain used in fed-batch processes. The strain is completely blocked in its ability to convert pyruvate into acetyl-CoA or acetate (using glucose as the carbon source) resulting in an acetate auxotrophy during growth in glucose minimal medium. Parameter estimation was carried out using data from fed-batch fermentation performed at constant glucose feed rates of q VG=10 mL h⁻¹. Acetate was fed according to the previously developed feeding strategy. While the model identification was realized by least-square fit, the model discrimination was based on the model selection criterion (MSC). The validation of model parameters was performed applying data from two different fed-batch experiments with glucose feed rate q VG=20 and 30 mL h⁻¹, respectively. Consequently, the most suitable model was identified that reflected the pyruvate and biomass curves adequately by considering a pyruvate inhibited growth (Jerusalimsky approach) and pyruvate inhibited product formation (described by modified Luedeking–Piret/Levenspiel term).