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Isolation and expression analysis of four members of the plasma membrane H+-ATPase gene family in Hevea brasiliensis

Zhu, Jiahong, Xu, Jing, Chang, Wenjun, Zhang, Zhili
Trees 2015 v.29 no.5 pp. 1355-1363
H-transporting ATP synthase, Hevea brasiliensis, ethylene, flowers, gene expression, gene expression regulation, genes, latex, leaves, metabolism, methyl jasmonate, phylogeny, plasma membrane, promoter regions, roots, stress response
KEY MESSAGE : Four PM H ⁺ -ATPase genes from Hevea brasiliensis were identified, and their structure, phylogenetic relationships, expression patterns and roles in latex metabolism were analyzed. Plasma membrane (PM) H⁺-ATPases play important roles in various cellular processes and stress responses. However, information on the PM H⁺-ATPase gene family of Hevea brasiliensis is limited. In this study, four PM H⁺-ATPase (HbHA) genes (HbHA1–4) were identified in H. brasiliensis. The four HbHAs were found to share high identity with PM H⁺-ATPases from other plant species and were classified into subfamilies II, IV, and V. Several cis-elements related to hormone, stress, growth, and development signals were found in the putative promoter regions of these HbHAs. Gene expression results showed that the four HbHAs were differentially expressed in the roots, barks, flowers, leaves, and latex of H. brasiliensis, with HbHA2 and HbHA3 strongly expressed in the latex. All HbHAs were upregulated by methyl jasmonate (MeJA) treatment. HbHA1 was repressed by ethylene (ET) treatment, whereas HbHA2, HbHA3 and HbHA4 were induced by ET. These results suggested that HbHAs may be involved in the regulation of latex metabolism in H. brasiliensis.