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Detection and quantification of rRNA by high-resolutionin situ hybridization in pollen grains

Author:
Saito, Chieko, Fujie, Makoto, Sakai, Atsushi, Kuroiwa, Haruko, Kuroiwa, Tsuneyoshi
Source:
Journal of plant research 1998 v.111 no.1 pp. 45-52
ISSN:
0918-9440
Subject:
Nicotiana tabacum, in situ hybridization, pollen, quantitative analysis, ribosomal RNA
Abstract:
We examined changes in the localization of cytoplasmic rRNA during pollen development inNicotiana tabacum SR-1. The rRNA was visualized byin situ hybridization, and the signal intensity of rRNA in microspore, vegetative and generative cell was quantified by microphotometry. The amount of rRNA per microspore or pollen section increased about 5 times from microspore to mature pollen grain and kept increasing even in the late stage of pollen development after PMI. The increase of rRNA occur in both vegetative and generative cells. The results suggest that synthesis of rRNA occur even after PM I in both vegetative and generative cells.
Agid:
4391421