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Determination of vitamins D2 and D3 in pharmaceuticals by supercritical-fluid extraction and HPLC separation with UV detection

Author:
Gámiz-Gracia, L., Jiménez-Carmona, M. M., Luque de Castro, M. D.
Source:
Chromatographia 2000 v.51 no.7-8 pp. 428-432
ISSN:
0009-5893
Subject:
carbon dioxide, drugs, ethyl ether, high performance liquid chromatography, reversed-phase liquid chromatography, vitamin D
Abstract:
The supercritical-fluid extraction of vitamins D₂ and D₃ with carbon dioxide is reported for the first time. The extraction recovery was enhanced by direct addition of diethyl ether to sample contained in the extraction cell. Separation and detection of the analytes was performed off-line by reversed-phase liquid chromatography with UV-detection. The quantification limit of the method is 4.1 μg for both analytes, with precision, expressed as relative standard deviation, of 3.8 and 6.3% for vitamins D₂ and D₃, respectively (η=7). The proposed method has been applied to the determination of vitamin D in different pharmaceutical products; recoveries were between 85 and 105%.
Agid:
4392013