Jump to Main Content
Optimization of the mobile phase conditions for the purification ofTaql endonuclease
- Düzenli, M., Ülgen, K. Ö.
- Chromatographia 2000 v.52 no.5-6 pp. 377-380
- anion exchange, buffers, cation exchange, high performance liquid chromatography, histidine, pH, phosphates, sodium acetate
- Taql endonuclease was purified by high performance ion exchange liquid chromatography with linear gradient elution. Of the chromatographic media tested as mobile phases for the HPLC purification ofTaql endonuclease, sodium acetate (pH 5.0) and phosphate (pH 7.0) buffers were appropriate for use with cation exchange columns, and L-histidine (pH 6.0) and Tris (pH 8.0) buffers with anion exchange columns.