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Optimization of the mobile phase conditions for the purification ofTaql endonuclease

Author:
Düzenli, M., Ülgen, K. Ö.
Source:
Chromatographia 2000 v.52 no.5-6 pp. 377-380
ISSN:
0009-5893
Subject:
anion exchange, buffers, cation exchange, high performance liquid chromatography, histidine, pH, phosphates, sodium acetate
Abstract:
Taql endonuclease was purified by high performance ion exchange liquid chromatography with linear gradient elution. Of the chromatographic media tested as mobile phases for the HPLC purification ofTaql endonuclease, sodium acetate (pH 5.0) and phosphate (pH 7.0) buffers were appropriate for use with cation exchange columns, and L-histidine (pH 6.0) and Tris (pH 8.0) buffers with anion exchange columns.
Agid:
4392053