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N-Acetyl-β-D-hexosaminidase Secreted by the Marine Fungus Phoma glomerata
- Zhuravleva, N. V., Luk'yanov, P. A., Pivkin, M. V.
- Applied biochemistry and microbiology 2004 v.40 no.5 pp. 448-453
- Phoma glomerata, beta-N-acetylhexosaminidase, biosynthesis, gel chromatography, ion exchange chromatography, marine fungi, microbial growth, molecular weight, mycelium, polyacrylamide gel electrophoresis
- Among the ten strains of marine fungi studied, the mycelial fungus Phoma glomerata showed maximum potency in producing N-acetyl-β-D-glucosaminidase. The conditions for fungal growth and enzyme biosynthesis were evaluated. N-Acetyl-β-D-hexosaminidase was isolated from the culture liquid of Phoma glomerata by ion-exchange chromatography (on DEAE–cellulose and DEAE–Sephacell) and gel filtration (on Toyopearl HW-55) with a yield of 35%; the enzyme, purified 36.4-fold, had a molecular weight of 20 kDa. The homogeneity of the enzyme was confirmed by gel filtration and SDS-PAGE. Transglycosylation reactions catalyzed by the enzyme produced N-acetyl-D-glucosamine and N-acetyl-D-galactosamine with respective yields of 38 and 46%.