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Chemical characterization of airborne bacteria using X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIRS)

Author:
Pastuszka, Jozef S., Talik, Ewa, Hacura, Andrzej, Słoka, Joanna, Wlazło, Agnieszka
Source:
Aerobiologia 2005 v.21 no.3-4 pp. 181-192
ISSN:
0393-5965
Subject:
Fourier transform infrared spectroscopy, X-radiation, X-ray photoelectron spectroscopy, airborne microorganisms, sampling, strains
Abstract:
In this study, the chemical characterization of airborne bacteria is explored using X-ray photoelectron spectrometry (XPS) and Fourier transform infrared spectroscopy (FTIRS). Samples were taken by using a 6-stage Andersen impactor. Airborne bacteria were collected on nutrient media in Petri dishes located on all impactor stages. On the basis of the obtained XPS spectra the ratios of N/C, O/C, and P/C were calculated for 11 Gram-positive and 4 Gram-negative strains. The results agree well with the data obtained by others for the test microorganisms/stock cultures. It is concluded that XPS analysis might be applied for identification of airborne bacterial strains and species but rather in conjunction with different techniques. Especially, a complementary method to XPS seems to be FTIRS.
Agid:
4392969