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Paenibacillus populi sp. nov., a novel bacterium isolated from the rhizosphere of Populus alba

Author:
Han, Tong-Yan, Tong, Xiao-Mei, Wang, Yan-Wei, Wang, Hui-Min, Chen, Xiao-Rong, Kong, De-Long, Guo, Xiang, Ruan, Zhi-Yong
Source:
Antonie van Leeuwenhoek 2015 v.108 no.3 pp. 659-666
ISSN:
0003-6072
Subject:
DNA, Paenibacillus, Populus alba, bacteria, cell walls, chemotaxonomy, fatty acids, menaquinones, new species, nucleic acid hybridization, nucleotide sequences, pH, peptidoglycans, phosphatidylethanolamines, phylogeny, rhizosphere, ribosomal RNA, sequence homology, sodium chloride, temperature
Abstract:
A novel aerobic bacterium, designated strain LAM0705ᵀ, was isolated from the rhizosphere of Populus alba in the Peking University Third Hospital. Cells of strain LAM0705ᵀ were observed to be Gram-stain positive, motile, spore-forming and rod-shaped. The optimal temperature and pH for growth were found to be 30 °C and pH 7.5, respectively. Strain LAM0705ᵀ was found to be able to grow in the presence 0–5 % NaCl (w/v) (optimum 1.0 %). The major fatty acids of strain LAM0705ᵀ were identified as anteiso-C₁₅:₀, C₁₆:₀ and iso-C₁₆:₀. The dominant polar lipids were found to consist of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The cell wall peptidoglycan of strain LAM0705ᵀ was found to contain meso-diaminopimelic acid. The predominant menaquinone was identified as MK-7. The G+C content of genomic DNA was found to be 48 mol% when determined by the T ₘ method. The 16S rRNA gene sequence similarity analysis indicated that strain LAM0705ᵀ is closely related to Paenibacillus agaridevorans DSM 1355ᵀ and Paenibacillus thailandensis KCTC 13043ᵀ with 97.8 and 96.1 % sequence similarity, respectively. The DNA–DNA hybridization value between strain LAM0705ᵀ and P. agaridevorans DSM 1355ᵀ was 47 ± 0.8 %. On the basis of its phenotypic, phylogenetic and chemotaxonomic characteristics, strain LAM0705ᵀ is concluded to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus populi sp. nov. is proposed. The type strain is LAM0705ᵀ (=ACCC 06427ᵀ = JCM 19843ᵀ).
Agid:
4393094