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Paenibacillus populi sp. nov., a novel bacterium isolated from the rhizosphere of Populus alba

Han, Tong-Yan, Tong, Xiao-Mei, Wang, Yan-Wei, Wang, Hui-Min, Chen, Xiao-Rong, Kong, De-Long, Guo, Xiang, Ruan, Zhi-Yong
Antonie van Leeuwenhoek 2015 v.108 no.3 pp. 659-666
DNA, Paenibacillus, Populus alba, bacteria, cell walls, chemotaxonomy, fatty acids, menaquinones, new species, nucleic acid hybridization, nucleotide sequences, pH, peptidoglycans, phosphatidylethanolamines, phylogeny, rhizosphere, ribosomal RNA, sequence homology, sodium chloride, temperature
A novel aerobic bacterium, designated strain LAM0705ᵀ, was isolated from the rhizosphere of Populus alba in the Peking University Third Hospital. Cells of strain LAM0705ᵀ were observed to be Gram-stain positive, motile, spore-forming and rod-shaped. The optimal temperature and pH for growth were found to be 30 °C and pH 7.5, respectively. Strain LAM0705ᵀ was found to be able to grow in the presence 0–5 % NaCl (w/v) (optimum 1.0 %). The major fatty acids of strain LAM0705ᵀ were identified as anteiso-C₁₅:₀, C₁₆:₀ and iso-C₁₆:₀. The dominant polar lipids were found to consist of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The cell wall peptidoglycan of strain LAM0705ᵀ was found to contain meso-diaminopimelic acid. The predominant menaquinone was identified as MK-7. The G+C content of genomic DNA was found to be 48 mol% when determined by the T ₘ method. The 16S rRNA gene sequence similarity analysis indicated that strain LAM0705ᵀ is closely related to Paenibacillus agaridevorans DSM 1355ᵀ and Paenibacillus thailandensis KCTC 13043ᵀ with 97.8 and 96.1 % sequence similarity, respectively. The DNA–DNA hybridization value between strain LAM0705ᵀ and P. agaridevorans DSM 1355ᵀ was 47 ± 0.8 %. On the basis of its phenotypic, phylogenetic and chemotaxonomic characteristics, strain LAM0705ᵀ is concluded to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus populi sp. nov. is proposed. The type strain is LAM0705ᵀ (=ACCC 06427ᵀ = JCM 19843ᵀ).