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Hydrogenophaga luteola sp. nov. isolated from reed pond water

Du, Juan, Yang, Jung-Eun, Singh, Hina, Akter, Shahina, Won, KyungHwa, Yin, Chang Shik, Jin, Feng-Xie, Yi, Tae-Hoo
Antonie van Leeuwenhoek 2015 v.108 no.3 pp. 695-701
DNA, Hydrogenophaga flava, Hydrogenophaga pseudoflava, bacteria, chemotaxonomy, color, fatty acids, isoprenoids, new species, nucleic acid hybridization, nucleotide sequences, phosphatidylethanolamines, ribosomal RNA, sequence analysis, China
A yellowish colored, Gram-staining negative, strictly aerobic, motile, rod-shaped bacterium, designated THG-SQE7ᵀ, was isolated from reed pond water in Shangqiu, PR China. Comparative 16S rRNA gene sequence analysis indicated that strain THG-SQE7ᵀ is most closely related to Hydrogenophaga pseudoflava ATCC 33668ᵀ (98.4 %), followed by Hydrogenophaga bisanensis K102ᵀ (97.6 %) and Hydrogenophaga flava CCUG 1658ᵀ (97.6 %). DNA–DNA hybridization showed 53.5, 36.0 and 22.5 % DNA re-association with H. pseudoflava KCTC 2348ᵀ, H. bisanensis KCTC 12980ᵀ and H. flava KCTC 1648ᵀ, respectively. Chemotaxonomic data revealed that strain THG-SQE7ᵀ possesses ubiquinone-8 as the only isoprenoid quinone, summed feature 3 (C₁₆:₁ ω7c and/or C₁₆:₁ ω6c), C₁₆:₀ and C₁₈:₁ ω7c as the major fatty acids. The major polar lipids were found to be phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was determined to be 63.7 mol%. These data corroborated the affiliation of strain THG-SQE7ᵀ to the genus Hydrogenophaga. Thus, the isolate represents a novel species, for which the name Hydrogenophaga luteola sp. nov. is proposed, with THG-SQE7ᵀ as the type strain (=KCTC 42501ᵀ = CCTCC AB 2014314ᵀ = JCM 30433ᵀ).