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Paenibacillus herberti sp. nov., an endophyte isolated from Herbertus sendtneri

Guo, Guan Nan, Zhou, Xun, Zhao, Ran, Chen, Xin Yao, Chen, Zhi Ling, Li, Xue Dong, Li, Yan Hong
Antonie van Leeuwenhoek 2015 v.108 no.3 pp. 587-596
DNA, Paenibacillus, alcohols, bacteria, chemotaxonomy, endophytes, fatty acids, genes, isoprenoids, new species, nucleotide sequences, peptidoglycans, phosphatidylethanolamines, phylogeny, ribosomal RNA, sequence homology
Strain R33ᵀ, an endophyte recovered from Herbertus sendtneri, was identified as representing a novel species of the genus Paenibacillus by using a polyphasic taxonomic approach. The novel strain was observed to be a Gram-stain positive, aerobic, rod-shaped, motile and endospore-forming bacterium. The major polar lipids of strain R33ᵀ were identified as diphosphatidylglycerol, phosphatidylethanolamine, along with lesser amounts of phosphatidylglycerol, three unidentified aminophospholipids, two unidentified phospholipids and two unidentified lipids. The predominant isoprenoid quinone was identified as MK-7. The major fatty acids (>8.0 %) were found to be anteiso-C₁₅:₀ (40.0 %), C₁₆:₁ ω11c (9.4 %), C₁₆:₁ ω7c alcohol (8.5 %) and C₁₆:₀ (8.2 %). The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid. The G+C content of genomic DNA was determined to be 56.9 mol%. The 16S rRNA gene sequence similarities of strain R33ᵀ to other Paenibacillus species ranged from 91.6 to 97.2 %, with high similarities to Paenibacillus humicus PC-147ᵀ and Paenibacillus pasadenensis SAFN-007ᵀ. The phylogenetic analyses based on 16S rRNA gene sequences and the partial rpoB gene confirmed that strain R33ᵀ belongs to the genus Paenibacillus. However, strain R33ᵀ shows differential molecular characteristics compared to other related Paenibacillus species based on 16S rDNA-RFLP analyses; the DNA–DNA relatedness values between strain R33ᵀ and P. humicus PC-147ᵀ, and that between strain R33ᵀ and P. pasadenensis SAFN-007ᵀ, were 35.0 ± 2.0 and 41.4 ± 0.9 %, respectively. Based on its phenotypic, chemotaxonomic and phylogenetic properties, strain R33ᵀ is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus herberti is proposed (type strain R33ᵀ = CGMCC 1.15042ᵀ = DSM 29849ᵀ).