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Oceanobacillus damuensis sp. nov. and Oceanobacillus rekensis sp. nov., isolated from saline alkali soil samples

Long, Xiufeng, Ye, Renyuan, Zhang, Shuai, Liu, Bo, Zhang, Yuqin, Zeng, Zhigang, Tian, Yongqiang
Antonie van Leeuwenhoek 2015 v.108 no.3 pp. 731-739
aerobes, chemotaxonomy, fatty acids, isoprenoids, new species, nucleic acid hybridization, nucleotide sequences, pH, phosphatidylcholines, phosphatidylethanolamines, phylogeny, ribosomal RNA, soil sampling, China
Two moderately halophilic strains, PT-11ᵀ and PT-20ᵀ, were isolated from saline alkali soil samples collected in Shache County, Xinjiang Province, China. Both strains are aerobic, Gram-positive, motile rods. Strain PT-11ᵀ grows at 15–40 °C and at pH 6.5–10.0, while PT-20ᵀ grows at 15–40 °C and at pH 6.5–11.0. The major cellular fatty acids in both strains include anteiso-C₁₅:₀, anteiso-C₁₇:₀ and iso-C₁₅:₀. For both strains, the polar lipids consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and several unidentified lipids. In addition, strain PT-20ᵀ also contains phosphatidylcholine. The major isoprenoid quinone for both strains is MK-7. The genomic G+C content is 36.7 % for PT-11ᵀ and 39.2 % for PT-20ᵀ. Phylogenetic analyses of 16S rRNA gene sequences indicated that these two isolates are members of the genus Oceanobacillus. DNA–DNA hybridization indicated that strains PT-11ᵀ and PT-20ᵀ should be considered two distinct species. On the basis of both phylogenetic and chemotaxonomic data analyses, therefore, we conclude that PT-11ᵀ and PT-20ᵀ represent two novel species within the genus Oceanobacillus, for which we propose the names Oceanobacillus rekensis sp. nov. and Oceanobacillus damuensis sp. nov., respectively. The type strains are PT-11ᵀ (=KCTC 33144ᵀ = DSM 26900ᵀ) and PT-20ᵀ (=KCTC 33146ᵀ = DSM 26901ᵀ).