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Oceanobacillus damuensis sp. nov. and Oceanobacillus rekensis sp. nov., isolated from saline alkali soil samples

Author:
Long, Xiufeng, Ye, Renyuan, Zhang, Shuai, Liu, Bo, Zhang, Yuqin, Zeng, Zhigang, Tian, Yongqiang
Source:
Antonie van Leeuwenhoek 2015 v.108 no.3 pp. 731-739
ISSN:
0003-6072
Subject:
aerobes, chemotaxonomy, fatty acids, isoprenoids, new species, nucleic acid hybridization, nucleotide sequences, pH, phosphatidylcholines, phosphatidylethanolamines, phylogeny, ribosomal RNA, soil sampling, China
Abstract:
Two moderately halophilic strains, PT-11ᵀ and PT-20ᵀ, were isolated from saline alkali soil samples collected in Shache County, Xinjiang Province, China. Both strains are aerobic, Gram-positive, motile rods. Strain PT-11ᵀ grows at 15–40 °C and at pH 6.5–10.0, while PT-20ᵀ grows at 15–40 °C and at pH 6.5–11.0. The major cellular fatty acids in both strains include anteiso-C₁₅:₀, anteiso-C₁₇:₀ and iso-C₁₅:₀. For both strains, the polar lipids consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and several unidentified lipids. In addition, strain PT-20ᵀ also contains phosphatidylcholine. The major isoprenoid quinone for both strains is MK-7. The genomic G+C content is 36.7 % for PT-11ᵀ and 39.2 % for PT-20ᵀ. Phylogenetic analyses of 16S rRNA gene sequences indicated that these two isolates are members of the genus Oceanobacillus. DNA–DNA hybridization indicated that strains PT-11ᵀ and PT-20ᵀ should be considered two distinct species. On the basis of both phylogenetic and chemotaxonomic data analyses, therefore, we conclude that PT-11ᵀ and PT-20ᵀ represent two novel species within the genus Oceanobacillus, for which we propose the names Oceanobacillus rekensis sp. nov. and Oceanobacillus damuensis sp. nov., respectively. The type strains are PT-11ᵀ (=KCTC 33144ᵀ = DSM 26900ᵀ) and PT-20ᵀ (=KCTC 33146ᵀ = DSM 26901ᵀ).
Agid:
4393103