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Lysobacter agri sp. nov., a bacterium isolated from soil

Singh, Hina, Won, KyungHwa, Du, Juan, Yang, Jung-Eun, Akter, Shahina, Kim, Ki-Young, Yi, Tae-Hoo
Antonie van Leeuwenhoek 2015 v.108 no.3 pp. 553-561
Lysobacter enzymogenes, bacteria, chemical structure, fatty acids, nucleotide sequences, pH, phosphatidylethanolamines, phylogeny, ribosomal RNA, sequence homology, sodium chloride, soil, ubiquinones, South Korea
A bacterial strain, designated as THG-SKA3ᵀ, was isolated from field soil of Kyung Hee University, South Korea. Cells of the isolate were observed to be Gram-negative, aerobic, rod-shaped and motile by gliding. The strain was found to grow optimally at 28 °C, at pH 7 and in absence of NaCl. Based on 16S rRNA gene sequence comparisons, strain THG-SKA3ᵀ shared highest sequence similarity with Lysobacter niastensis KACC 11588ᵀ followed by Lysobacter panacisoli KACC 17502ᵀ, Lysobacter enzymogenes LMG 8762ᵀ and Lysobacter oryzae KCTC 22249ᵀ. The G+C content of THG-SKA3ᵀ was determined to be 68.9 mol%. The DNA–DNA relatedness values between strain THG-SKA3ᵀ and its closest phylogenetic neighbors were below 25.0 %.The major polar lipids of strain THG-SKA3ᵀ were determined to be diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant respiratory quinone was identified as ubiquinone 8 (Q-8). The major cellular fatty acids were identified as branched chain iso-C₁₅:₀, iso-C₁₆:₀ and unsaturated iso-C₁₇:₁ ω9c. On the basis of polyphasic data presented, it is evident that strain THG-SKA3ᵀ represents a novel species of the genus Lysobacter, for which the name Lysobacter agri sp. nov. (type strain THG-SKA3ᵀ = KACC 18283ᵀ = CSCTCC AB 2015126ᵀ) is proposed.