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The Decrease of Extracted Apoplast Protein in Soybean Root Tip by Aluminium Treatment

T. Kataoka, J. Furukawa, T.M. Nakanishi
Biologia plantarum 2003 v.46 no.3 pp. 445-449
Glycine max, aluminum, apoplast, magnesium chloride, polyacrylamide gel electrophoresis, root tips, seedlings, soy protein, soybeans, two-dimensional gel electrophoresis
Aluminium effect on the mobility of apoplast protein in root tips was studied. Two-day seedlings of soybean (Glycine max. (L.) Merr. cv. Tsurunoko) were treated with 50 μM AlCl₃ for 2 h. Using infiltration method, the apoplast protein in root tips was extracted with 20 or 100 mM MgCl₂. When 20 mM MgCl₂ was used to collect weakly bound protein to apoplast, the amount of protein extracted was reduced to be about 20 % compared with that of control and the band of 97 kDa disappeared in SDS-PAGE gel. However, the 97 kDa protein could be extracted by 100 mM MgCl₂, which were used for extraction of more tightly bound protein to apoplast, and the amount was estimated to be the same as that of control. When the protein was further developed in two-dimensional electrophoresis, three spots were found between pI 6.4 and 6.5. This is the first report of an Al effect on the mobility of apoplast protein.