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A simple practical method for bulk and rapid extraction of free-living nematodes from marine and estuarine sediments

Mangubhai, Sangeeta, Greenwood, Jack G.
Hydrobiologia 2004 v.522 no.1-3 pp. 343-347
Nematoda, estuarine sediments, filtration, free-living nematodes, marine sediments, paper, rapid methods, temperature
A simple, rapid method is described for the extraction of large numbers of free-living nematodes from estuarine sediments. This method does not physically or chemically alter or damage the nematodes, but instead relies on their downward movement through a filtering layer of double ply tissue paper and into aerated water-filled trays. Seven trials each with 10 trays kept at 25 °C for an initial period of 24 h yielded 3985 live nematodes l⁻¹ (± 511.5 standard deviation) of estuarine sediment, free of sediment and with minimal debris. Time effects were statistically significantly different, with the same 10 trays yielding another 1259 nematodes l⁻¹ (± 413.4) when kept for a second period of 24 h at the same temperature. Temperature effects were also significant, and 7 trials each with 10 trays kept for 24 h at 20–21 °C, produced a lower yield of 2160 nematodes l⁻¹ (± 532.7) of sediment. The method is expected to be of use in nematode extractions from both estuarine and marine sediments.