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Effects of additives on the lyophilized and thermal stability of D-galactose-6-sulfurylase activity from Eucheuma striatum (Rhodophyta)

Author:
Qin, Xiaojuan, Ma, Chaoyang, Lou, Zaixiang, Wang, Hongxin
Source:
Journal of applied phycology 2015 v.27 no.4 pp. 1709-1715
ISSN:
0921-8971
Subject:
Eucheuma, additives, carrageenan, enzyme activity, freeze drying, glycerol, glycine (amino acid), mannitol, sucrose, thermal stability, trehalose
Abstract:
D-galactose-6-sulfurylase has potential application in the improvement of the carrageenan quality. The lyophilized and thermal stability of the Gal-6-sulfurylase prepared from Eucheuma striatum was investigated using selected chemical additives. The addition of 5 % (w/v) sucrose and trehalose to Gal-6-sulfurylase prior to lyophilization could retain nearly 100 % enzymatic activity, compared to only 19 % for the lyophilized control. The lyophilized Gal-6-sulfurylase containing sucrose maintained full activity for even 40 days storage at −20 °C. For Gal-6-sulfurylase solution, glycerol was effective for long-term stability at −20 °C. Moreover, polyol sucrose and amino acid glycine enhanced the thermal stability of Gal-6-sulfurylase, while polyol mannitol decreased the thermal stability of Gal-6-sulfurylase. The results show that the Gal-6-sulfurylase, found in the red alga E. striatum, was stabilized by the addition of chemical additives.
Agid:
4396758