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The new facultatively chemolithoautotrophic, moderately halophilic, sulfate-reducing bacterium Desulfovermiculus halophilus gen. nov., sp. nov., isolated from an oil field

Belyakova, E. V., Rozanova, E. P., Borzenkov, I. A., Tourova, T. P., Pusheva, M. A., Lysenko, A. M., Kolganova, T. V.
Microbiology 2006 v.75 no.2 pp. 161-171
DNA, Desulfohalobiaceae, acetates, alanine, butyrates, developmental stages, ethanol, fermentation, growth factors, malates, menaquinones, mineralization, new genus, new species, oil fields, pH, phylogeny, propionic acid, pyruvic acid, sodium chloride, succinic acid, sulfate-reducing bacteria, sulfates, sulfur, temperature, thiosulfates
The new mesophilic, chemolithoautotrophic, moderately halophilic, sulfate-reducing bacterium strain 11-6, could grow at a NaCl concentration in the medium of 30–230 g/l, with an optimum at 80–100 g/l. Cells were vibrios motile at the early stages of growth. Lactate, pyruvate, malate, fumarate, succinate, propionate, butyrate, crotonate, ethanol, alanine, formate, and H₂/CO₂ were used in sulfate reduction. Butyrate was degraded completely, without acetate accumulation. In butyrate-grown cells, a high activity of CO dehydrogenase was detected. Additional growth factors were not required. Autotrophic growth occurred, in the presence of sulfate, on H₂/CO₂ or formate without other electron donors. Fermentation of pyruvate and fumarate was possible in the absence of sulfate. Apart from sulfate, sulfite, thiosulfate, and elemental sulfur were able to serve as electron acceptors. The optimal growth temperature was 37°C; the optimum pH was 7.2. Desulfoviridin was not detected. Menaquinone MK-7 was present. The DNA G+C content was 55.2 mol %. Phylogenetically, the bacterium represented a separate branch within the cluster formed by representatives of the family Desulfohalobiaceae in the class Deltaproteobacteria. The bacterium was assigned to a new genus and species, Desulfovermiculus halophilus gen. nov., sp. nov. The type strain is 11-6ᵀ (= VKM B-2364), isolated from the highly mineralized formation water of an oil field.