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On the constructing of survival curves for cultured cells in cytogerontological experiments: A brief note with three hierarchy diagrams
- Khokhlov, A. N., Morgunova, G. V.
- Moscow University biological sciences bulletin 2015 v.70 no.2 pp. 67-71
- cell viability, cultured cells, models, senescence (aging)
- A brief comparative analysis of different approaches to cell viability testing in cytogerontological experiments is performed with a focus on problems in constructing survival curves for cultured cells in the “stationary phase aging” model. It is emphasized that the choice of methods to this end depends mainly on the researchers’ ideas about molecular and cellular mechanisms of aging. A note is made that the evaluation of colonyforming efficiency, though optimal for cell viability assessment, is unfortunately not applicable to postmitotic or very slowly propagating cells. Consideration is also given to some problems encountered when using the most popular molecular probes designed for live/dead cell viability assays.