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Development of Sandwich ELISA and Immunochromatographic Strip for the Detection of Peanut Allergen Ara h 2

Author:
Peng, Juan, Song, Shanshan, Liu, Liqiang, Kuang, Hua, Xu, Chuanlai
Source:
Food analytical methods 2015 v.8 no.10 pp. 2605-2611
ISSN:
1936-9751
Subject:
allergens, antibody detection, cross reaction, detection limit, enzyme-linked immunosorbent assay, immunoaffinity chromatography, monoclonal antibodies, peanuts
Abstract:
In order to develop highly sensitive immunoassays for detecting the major peanut allergen, Ara h 2, we prepared six monoclonal antibodies (mAb) with high affinity to Ara h 2. Using mAb 5 as a capture antibody and HRP-labeled mAb 1 as a detection antibody, a sandwich enzyme-linked immunoassay (ELISA) was established with a limit of detection (LOD) of 0.02 ng/mL. An immunochromatographic strip based on these two mAbs was also established with a LOD of 1 ng/mL. Both tests showed high specificity in cross-reactivity experiments. Sample analyses indicated that these two immunoassays were feasible, accurate, and highly sensitive for measuring allergen Ara h 2 of food.
Agid:
4404107