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Cytokinin profiling of long-term in vitro pea (Pisum sativum L.) shoot cultures
- Plačková, Lenka, Hrdlička, Jakub, Smýkalová, Iva, Cvečková, Magdalena, Novák, Ondřej, Griga, Miroslav, Doležal, Karel
- Plant growth regulation 2015 v.77 no.2 pp. 125-132
- Pisum sativum, cytokinins, genetic stability, growth and development, metabolites, peas, plantlets, rooting, sampling, shoots
- Forty-seven endogenous cytokinin metabolites were determined in samples of long-term in vitro multiple shoot cultures of pea (Pisum sativum L.). Significant differences were found in the levels of all cytokinin groups except cis-zeatin and some of its derivatives. Old cultures (30 or 10 years old) contained larger total and active cytokinin pools than freshly prepared cultures. Long-term shoot cultures maintained on cytokinin-supplemented media initially exhibit gradually increasing concentrations of endogenous cytokinins that subsequently stabilise at a high level, which can make it more difficult to induce rooting later on. This is consistent with previous findings concerning the growth and development of plantlets derived from long-term cultures and the generally accepted role of cytokinins during root development. The results presented herein demonstrate the utility of long-term pea cultures as model systems for studying the influence of long-term cultivation on genetic stability, and suggest ways of circumventing the rooting problems associated with such cultures.