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A simple and rapid procedure for identification of seed coat colour at the early developmental stage of Brassica juncea and Brassica napus seeds
- Lu, Ying, Liu, Xianjun, Liu, Shuyan, Yue, Yingchun, Guan, Chunyun, Liu, Zhongsong
- Plant breeding 2012 v.131 no.1 pp. 176-179
- Brassica juncea, Brassica napus, breeding, color, early development, flowering, pollination, rapeseed, seed maturation, seeds, testa, vanillin
- With 4 figures and 2 tables ABSTRACT: The yellow seed is a desirable trait in oilseed Brassica species. However, seed coat colour cannot be observed until the maturation of the seed. Identification of seed coat colour at early seed development will allow for the early detection of yellowâseeded individual plants in the segregating populations at the same generation and thus speed up yellowâseeded rapeseed breeding programme. Colour of seed coats from Brassica juncea and Brassica napus can precisely be determined as early as 15âdays after pollination by staining with 0.5% vanillinâHCl solution for 5âmin, which is shown to be simple, rapid and reliable. Flowering duration of an individual plant usually lasts for approximately 25â35âdays in Brassica species and so the seed coat colour of an individual plant can be determined 10â20âdays before the end of its flowering. Through this technique, breeding for the yellowâseeded trait can now be advanced one generation earlier by vanillin staining than by conventional visual observation of testa colour of mature Brassica seeds.