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15α-Hydroxylation of a steroid (13-ethyl-gon-4-en-3,17-dione) by Penicillium raistrickii in an ionic liquid/aqueous biphasic system
- Mao, Shuhong, Hu, Xiaojie, Hua, Boyuan, Wang, Na, Liu, Xiaoguang, Lu, Fuping
- Biotechnology letters 2012 v.34 no.11 pp. 2113-2117
- Penicillium, biotransformation, industrial applications, ionic liquids, pH, water solubility
- Biphasic processes are used in whole-cell biotransformation to overcome the low water solubility of substrates and products as well as their inhibitory effects on the biocatalyst. Commercially available [NTf2]- and [PF6]-based ionic liquids (ILs) were used in a biphasic system for the 15α-hydroxylation of 13-ethyl-gon-4-en-3,17-dione by Penicillium raistrickii. With the substrate at 5 g l−1 and a volume ratio of IL to buffer, buffer pH and cell density at, 1:9, 6.5, 16.8 gDW l−1, respectively, the 15α-hydroxylation of 13-ethyl-gon-4-en-3,17-dione was achieved with a yield of 70 % after 72 h using [BMIm][NTf2] in a 50 ml biphasic system. This is compared to a 30 % yield in a monophasic aqueous system. This suggests the potential industrial application of ILs-based biphasic systems for steroid biotransformation.