PubAg

Main content area

Platelet thrombus formation and hemostasis are delayed in the microcirculation of copper-deficient rats

Author:
Schuschke, Dale A., Saari, Jack T., Nuss, James W., Miller, Frederick N.
Source:
Journal of nutrition 1994 v.124 no.8 pp. 1258
ISSN:
0022-3166
Subject:
copper, nutrient deficiencies, dietary minerals, hemostasis, blood coagulation, nutritional adequacy, heart rate, blood pressure, liver, blood plasma, thromboplastin, prothrombin, arteries, rats, blood platelets, hematocrit
Abstract:
The role of dietary copper in platelet thrombus formation and hemostasis was studied in the cremaster muscle microcirculation. Male weanling Sprague-Dawley rats were fed purified diets that were either copper-adequate (94 micromole Cu/kg diet) or copper-deficient (0 micromole Cu/kg diet) for 1, 3 or 5 wk. The rats were anesthetized with pentobarbital, and the cremaster was spread in a Krebs-filled tissue bath. Fluorescein isothiocyanate tagged to bovine serum albumin (FITC-BSA) was injected intra-arterially. After a 20-min equilibration, blue light (1.8 W/cm2, 450-490 nm) was used to activate the FITC-BSA and induce platelet thrombus formation within the vasculature. In vivo television microscopy was used to quantify the thrombus formation. In rats fed the copper-deficient diet for 3 or 5 wk, platelet thrombus formation induced by photoactivation was significantly (P < 0.05) delayed and prothrombin time was significantly longer but the number of circulating platelets was significantly greater than in age-matched rats fed the copper-adequate diet. Bleeding time, measured after micropuncture of a second-order venule, was significantly longer but hematocrit was significantly lower in rats fed the copper-deficient diet than in those fed the copper-adequate diet. The results demonstrate that platelet-mediated hemostasis is depressed in dietary copper deficiency and that this may be due to a decrease in hematocrit, a decrease in the activity of a coagulation factor and/or an alteration of platelet function.
Agid:
44222
Handle:
10113/44222