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Immunomodulatory and antioxidant potential of Lactobacillus exopolysaccharides

Author:
Liu, Chin‐Feng, Tseng, Kuo‐Chuan, Chiang, Shen‐Shih, Lee, Bao‐Hong, Hsu, Wei‐Hsuan, Pan, Tzu‐Ming
Source:
Journal of the science of food and agriculture 2011 v.91 no.12 pp. 2284-2291
ISSN:
0022-5142
Subject:
Lactobacillus paracasei subsp. paracasei, Lactobacillus plantarum, antioxidant activity, arabinose, cell proliferation, chelation, chromatography, emulsifiers, exopolysaccharides, food industry, food microbiology, free radical scavengers, fructose, galactose, glucose, ions, lactic acid bacteria, linoleic acid, mannose, peroxidation
Abstract:
BACKGROUND: Immunomodulation by probiotic microorganisms has become a topic of increasing interest in food microbiology. Polysaccharides are broadly used in the food industry as gelling, thickening, stabilizing, or emulsifying agents. Some probiotics such as lactic acid bacteria also produce exopolysaccharides that stimulate macrophage production of cytokines. The aim of this study was to characterize the effects of exopolysaccharides of Lactobacillus paracasei subsp. paracasei NTU 101 (101EP) and Lactobacillus plantarum NTU 102 (102EP) exopolysaccharides on antioxidant activity and immunomodulation in vitro. RESULTS: The sugar composition (including arabinose, galactose, glucose, fructose, mannose, and maltose) of 101EP and 102EP was quantified by high‐performance anion‐exchange chromatography. Cytokine production (including IL‐6, TNF‐α, and IL‐1β) was induced by 101EP and 102EP in Raw 264.7 in a dose‐dependent manner (5‐500 µg mL−1). 101EP and 102EP also demonstrated potential antioxidant properties (1,1‐diphenyl‐2‐picrylhydrazyl radical scavenging activity, chelation of ferrous ions, inhibition of linoleic acid peroxidation, and reducing power) in vitro. CONCLUSION: 101EP and 102EP stimulate cell proliferation and may be useful as a mild immune modulator of macrophages.
Agid:
444695