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Improvement of Catalytic Properties of Escherichia coli Penicillin G Acylase Immobilized on Glyoxyl Agarose by Addition of a Six-Amino-Acid Tag

Author:
Scaramozzino, Francesca, Estruch, Ilona, Rossolillo, Paola, Terreni, Marco, Albertini, Alessandra M.
Source:
Applied and environmental microbiology 2005 v.71 no.12 pp. 8937-8940
ISSN:
0099-2240
Subject:
Escherichia coli, agarose, amidase, benzylpenicillin
Abstract:
A tag of three lysines alternating with three glycines was added to the C-terminal end of the {szligbeta} chain of penicillin G acylase (PGA). This modification improved the immobilization efficiency of PGA on glyoxyl agarose and the catalytic properties of the PGA derivative, although it impaired the posttranslational steps of overexpressed protein maturation.
Agid:
445348