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Iron deprivation-induced reactive oxygen species generation leads to non-autolytic PCD in Brassica napus leaves

Tewari, Rajesh Kumar, Hadacek, Franz, Sassmann, Stefan, Lang, Ingeborg
Environmental and experimental botany 2013 v.91 pp. 74-83
Brassica napus, DNA fragmentation, NADP (coenzyme), catalase, cell death, chloroplasts, chromatin, dehydroascorbic acid, homeostasis, hydrogen peroxide, hydroponics, hydroxyl radicals, iron, leaves, photosystem II, superoxide anion, superoxide dismutase, thiobarbituric acid-reactive substances
Using iron-deprived (–Fe) chlorotic as well as green iron-deficient (5μM Fe) and iron-sufficient supplied (50μM Fe) leaves of young hydroponically reared Brassica napus plants, we explored iron deficiency effects on triggering programmed cell death (PCD) phenomena. Iron deficiency increased superoxide anion but decreased hydroxyl radical (OH) formation (TBARS levels). Impaired photosystem II efficiency led to hydrogen peroxide accumulation in chloroplasts; NADPH oxidase activity, however, remained on the same level in all treatments. Non-autolytic PCD was observed especially in the chlorotic leaf of iron-deprived plants, to a lesser extent in iron-deficient plants. It correlated with higher DNAse-, alkaline protease- and caspase-3-like activities, DNA fragmentation and chromatin condensation, hydrogen peroxide accumulation and higher superoxide dismutase activity. A significant decrease in catalase activity together with rising levels of dehydroascorbic acid indicated a strong disturbance of the redox homeostasis, which, however, was not caused by OH formation in concordance with the fact that iron is required to catalyse the Fenton reaction leading to OH generation. This study documents the chain of events that contributes to the development of non-autolytic PCD in advanced stages of iron deficiency in B. napus leaves.