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Bioguided fractionation and isolation of esculentoside P from Phytolacca americana L.
- Ding, Li-Juan, Ding, Wei, Zhang, Yong-Qiang, Luo, Jin-Xiang
- Industrial crops and products 2013 v.44 pp. 534-541
- Phytolacca americana, Tetranychus cinnabarinus, acaricidal properties, acaricides, acetone, adults, bioassays, fractionation, leaves, liquid chromatography, mass spectrometry, mortality, roots, silica gel, stems, thin layer chromatography
- Various solvent extracts from different organs of Phytolacca americana L. were tested for their acaricidal activities against female carmine spider mite [Tetranychus cinnabarinus (Boisduval)] adults by slide-dip bioassay. The acetone extracts of roots with mortality rates of 95.72% and 100.00% at 2.5mg/mL were more active than those of leaves and stems and compared with other solvent extracts from other organs after 48 and 72h of treatment, respectively. The root acetone extracts were fractionated using a silica gel column; after thin-layer chromatography, the concentrated extracts were separated into 15 groups and further analyzed for their acaricidal activities. The ninth fraction exhibited the highest acaricidal activity. Phytochemical evaluation and liquid chromatography/mass spectrometry analysis revealed that esculentoside P was the dominant active phytolaccasaponin in the said fraction. This biological compound is a promising candidate as an acaricide against T. cinnabarinus.