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Studies on triploid oysters in Australia: Evaluation of cytochalasin B and 6-dimethylaminopurine for triploidy induction in Sydney rock oysters Saccostrea commercialis (Iredale and Roughley)
- Nell, J.A., Hand, R.E., Goard, L.J., McAdam, S.P., Maguire, G.B.
- Aquaculture research 1996 v.27 no.9 pp. 689-698
- cytochalasin B, eggs, extrusion, larvae, oysters, spawning, triploidy, Australia
- Naturally spawned Sydney rock oysters Saccostrea commercialis (Iredale and Roughley),were used to determine the appropriate stage of development for inducing triploidy and to compare the effectiveness of cytochalasin B (CB) and 6-dimethylaminopurine (6-DMAP) in dose-optimization trials. Induction should commence at 50% first polar body (PB1) extrusion in eggs (approximately 17-19 min post-fertilization at 25oC). By day 5 the highest triploidy percentage and yield (number of triploid larvae per 100 fertilized eggs) were achieved in the ranges of 0.75-1.5 mg CB 1-¹ (1.6-3.1 μmCB)or 200-400 μm 6-DMAP (32.6-65.3 mg 6-DMAP l-¹). However, CB treatment resulted in greater survival and triploidy percentage than 6-DMAP in Sydney rock oysters.