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Cloning and characterization of a calcium dependent protein kinase gene associated with cotton fiber development

Author:
Huang, Quan-Sheng, Wang, Hai-Yun, Gao, Peng, Wang, Guo-Ying, Xia, Gui-Xian
Source:
Plant cell reports 2008 v.27 no.12 pp. 1869-1875
ISSN:
0721-7714
Subject:
Arabidopsis, calcium, calyx, genes, histones, leaves, lint cotton, onions, phosphorylation, plasma membrane, protein kinases, roots, trichomes
Abstract:
The gene GhCPK1 encoding a calcium dependent protein kinase was identified from cotton. Transcripts of GhCPK1 accumulated primarily in the elongating fiber, and Arabidopsis plants transformed with GhCPK1 promoter-GUS construct exhibited GUS activity mainly in the developing trichomes, roots, young leaves and sepals. In the bombarded onion epidermal cells, GhCPK1-GFP fusion proteins showed a subcellular distribution in the plasma membrane. In vitro assays indicated that GhCPK1 was a functional calcium-dependent kinase able to undergo autophosphorylation and phosphorylation of the known substrate histone III-S. Together, these results suggest that GhCPK1 may play a role in the calcium signaling events associated with fiber elongation.
Agid:
446883