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Ethanol production from Lycoris radiata Herbert (Amarylllidaceae) residues as a new resource
- Liu, Shuangping, Ding, Zhongyang, Zhang, Liang, Gu, Zhenghua, Wang, Xiaolan, Sun, Xiaojun, Shi, Guiyang
- Biomass and bioenergy 2012 v.37 pp. 237-242
- Lycoris radiata, Saccharomyces cerevisiae, alkaloids, alpha-amylase, batch fermentation, beta-glucanase, biotransformation, bulbs, cellobiose, crude fiber, crude protein, endo-1,4-beta-glucanase, ethanol, ethanol production, lignin, phytases, polygalacturonase, viscosity, xylanases, yeasts
- The large quantities of Lycoris radiata Herbert (Amarylllidaceae) residues, left after the extraction of alkaloids from the bulbs, could threaten the environment if not properly disposed. Therefore, the aim of this research is to investigate the feasibility of bioconversion of L. radiata Herbert residues to ethanol through batch fermentation. In L. radiata Herbert residues, the average contents (gÂ kgâ»Â¹) of non-structural carbohydrates, crude fiber, crude protein, ash, and lignin are 485.1, 177.3, 124.7, 108.9, and 91.0, respectively. Five commercial enzymes (Î²-glucanase, pectinase, xylanase, phytase and cellulase) were employed in pretreatment process and ethanol bioconversion was evaluated with three yeasts (Saccharomyces cerevisiae, osmotolerant S. cerevisiae and genetically engineered S. cerevisiae constructed to use cellobiose). The results showed that pretreament by Î²-glucanase successfully facilitated the penetration of Î±-amylase into ground material. After residues pretreatment by 10Â g kgâ»Â¹ of Î²-glucanase for 14Â h at 50Â Â°C, the viscosity decreased from 1135 to 59Â PaÂ·s which was equivalent to that obtained by the combined whole five enzymes. Further experiments proved that osmotolerant S. cerevisiae was desirable for ethanol production from Lycoris radiate Herbert residues. The results are helpful to develop non-grain bioethanol production.