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O-methyltransferase gene from sorghum cloning, expression, transformation and characterization

Author:
Baerson, Scott R., Rimando, Agnes M., Dayan, Franck E., Pan, Zhiqiang, Polashock, James J.
Source:
United States Department of Agriculture patents 2010 no.US 7,732,666 B1
Subject:
Sorghum bicolor, grain sorghum, genes, methyltransferases, molecular cloning, gene expression, genetic transformation, expressed sequence tags, root hairs, transgenic plants, enzyme activity, resveratrol, patents
Abstract:
This invention relates to an O-methyltransferase gene cloned from sorghum, the sorghum O-methyltransferase-3 gene, SbOMT3. Quantitative real-time RT-PCR and recombinant enzyme studies with putative O-methyltransferase sequences obtained from an EST data set from sorghum have led to the identification of the novel root hair-specific O-methyltransferase designated SbOMT3. Transgenic plants which express SbOMT3 can convert resveratrol into pterostilbene in planta. SbOMT3 is also involved in the biosynthesis of sorgoleone.
Agid:
44810
Handle:
10113/44810