Jump to Main Content
Optimization of a Rapid Viability Assay for Mycobacterium avium subsp. paratuberculosis by Using alamarBlue
- Carroll, James, Douarre, Pierre, Coffey, Aidan, Buckley, Jim, Cashman, Bill, O'Farrell, Kevin, O'Mahony, Jim
- Applied and environmental microbiology 2009 v.75 no.24 pp. 7870-7872
- Mycobacterium avium subsp. paratuberculosis, paratuberculosis, rifampicin, screening, viability
- A microtiter alamarBlue assay was adapted and optimized for Mycobacterium avium subsp. paratuberculosis. Using cell concentrations ranging from 10⁴ to 10⁸ CFU/ml, a minimum incubation time to indicate viability was obtained after 24 h. Rifampin (rifampicin) was used to demonstrate that this method has applications for high-throughput screening against M. avium subsp. paratuberculosis.