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Approved medication of water with enrofloxacin to treat turkey colibacillosis: Assessment of efficacy using a PK/PD approach

Russo, Elisa, Lucatello, Lorena, Giovanardi, Davide, Cagnardi, Petra, Ortali, Giovanni, Di Leva, Vincenzo, Montesissa, Clara
Veterinary microbiology 2012 v.161 no.1-2 pp. 206-212
Escherichia coli, blood sampling, drug therapy, enrofloxacin, flocks, fluoroquinolones, high performance liquid chromatography, oral administration, turkeys
The efficacy of administering enrofloxacin at 10mg/kg in medicated water to turkeys was evaluated by applying a PK/PD approach to the kinetic parameters obtained after oral pulsed administration and to the MIC values of avian pathogenic Escherichia coli (APEC) strains isolated from commercial turkey flocks. The kinetic parameters of enrofloxacin were evaluated in 10 healthy and 10 colisepticemic turkeys that received the drug dissolved in medicated water at 89μg/mL and 71μg/mL, respectively, for 10h/day for 5 days. Blood samples were collected for 24h from all turkeys on the last day of treatment, and the serum was analysed by HPLC with fluorimetric detection. The mean AUC (7374.53±1067.64hng/mL and 7656.95±1460.61hng/mL) and Cₘₐₓ values (673.09±186.18ng/mL and 543.50±68.75ng/mL) obtained for healthy and sick turkeys were not significantly different. High-level resistance was observed in 30.3% of strains, 40.5% exhibited intermediate resistance, and only 29.2% were susceptible; the MIC₅₀ and MIC₉₀ values were 1mg/L and 32mg/L, respectively. The PK/PD parameters Cₘₐₓ/MIC₅₀ (0.67 and 0.54 for healthy and sick turkeys, respectively) and AUC/MIC₅₀ (7.37 and 7.66) were lower than the efficacy breakpoints reported for fluoroquinolones. These results indicate that authorised dosage of enrofloxacin used in pulsed medicated water administration could be ineffective against more than the 70% of circulating APEC strains, indicating the need to test the drug susceptibility of APEC prior to administering the drug and adopting a more convenient medication schedule for mass treatment.