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A promising approach to enhance the total phenolic content and antioxidant activity of raw and processed Jatropha curcas L. kernel meal extracts

Srinivasan Nithiyanantham, Perumal Siddhuraju, George Francis
Industrial crops and products 2013 v.43 pp. 261-269
DNA, EDTA (chelating agent), Jatropha curcas, acetone, antioxidant activity, antioxidants, ascorbic acid, autoclaving, developing countries, drought, functional foods, iron, nitric oxide, seeds, trees
Jatropha curcas a member of the Euphorbiaceae family is a drought resistant small tree of great economic importance in developing countries because of its several industrial and medicinal values. The antioxidative properties, total phenolic content and DNA protection potential of raw and processed kernel meal extracts of Jatropha were analyzed. The samples were extracted with 70% acetone and used for the evaluation of its antioxidant potential. The total phenolic content of raw and processed Jatropha kernel meal samples ranged between 9.06 and 22.22mg TAN/g extract. The results of the study revealed that autoclaving and dry heat treatment causes a significant (P<0.05) increases in the total phenolic content and antioxidant properties. Nevertheless, raw samples exhibited a high metal chelating activity (0.97mg EDTA/g extract) compared to the processed samples. However, autoclaving with NaHCO3 and ash solution recorded the highest phenolic content (22.22mg TAN/g extract), FRAP (2861.11mmoL Fe(II)/g extract), phosphomolybdenum (353.61μmoL ascorbic acid/g extract), DPPH (6.29g extract/g DPPH), nitric oxide (55%), superoxide (67%) and hydroxyl radical (49%) scavenging activity when compared to the raw and other processed samples. The present studies may be of importance in varietal improvement, nutraceuticals and bio-pharmaceuticals as possible sources of cost-effective natural antioxidants.