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A strategy for detecting optimal ratio of cardioprotection-dependent three compounds as quality control of Guan-Xin-Er-Hao formula
- Wang, Yang, Huang, Xi, Qin, Feng, Ren, Ping, Zhu, Zhengyan, Fan, Rong, Zhang, Chunhu, Zhang, Hainan, Tang, Tao, He, Juan, Liu, Zhaoqian, Zhou, Honghao
- Journal of ethnopharmacology 2011 v.133 no.2 pp. 735-742
- apoptosis, bioactive properties, blood, caspase-3, ferulic acid, herbs, high performance liquid chromatography, infarction, myocardial infarction, quality control, rats
- AIMS: We aimed to detect optimal ratio of cardioprotection-dependent absorbed bioactive compounds (ABCs) as quality control of Guan-Xin-Er-Hao (GXEH) formula extracted by various processings. METHODS: Ferulic acid (F), tanshinol (T), hydroxysafflor yellow A (A), protocatechualdehyde (P) and paeoniflorin (E) in GXEH formula and FTA in blood from rat with acute myocardial infarction (AMI) were first identified by HPLC–MS/MS, and FTAPE in GXEH formulae with various herbs, extraction times and extraction water volumes were then quantitated only by HPLC. RESULTS: FTAPE in various GXEH were determined. FTA were selected as GXEH's ABCs. Ratios of FTA were determined, suggesting the high (1:6.1:15.6), medium (1:1.7:15.2) and low (1:0.2:15.3) ratios. Three FTA ratios and their parent formulae ratio-dependently reduced infarct size, myocardial apoptosis and caspase-3 activity. CONCLUSION: There is the optimal ratio of F:T:A among various formulae, contributing to the best cardioprotection. This FTA ratio was developed as quality control of GXEH formula.