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Evaluation of the Effect of 1,3-Bis(4-Phenyl)-1H-1,2,3-Triazolyl-2-Propanolol on Gene Expression Levels of JAK2–STAT3, NF-κB, and SOCS3 in Cells Cultured from Biopsies of Mammary Lesions

Author:
Becerril, J. L. Malvaez, Benítez, J. G. Santillán, Juárez, J. J. Torres, Bañales, J. M. González, Zerón, H. Mendieta, Navarro, M. D. Hernández
Source:
Biochemical genetics 2015 v.53 no.11-12 pp. 291-300
ISSN:
0006-2928
Subject:
biopsy, breast neoplasms, cell culture, cultured cells, gender, gene expression, inflammation, inhibitory concentration 50, non-specific protein-tyrosine kinase, overweight, patients, signal transduction, transcription factor NF-kappa B, waist circumference, women
Abstract:
Breast cancer is the most frequent neoplasia in women and is responsible for approximately 13.8% of deaths per year for this gender. It has been suggested that JAK2, STAT3, and NF-κB gene expression is involved in this type of cancer. The objective of the present study was to determine the effect of bistriazole in these signaling pathways in patients with breast cancer and benign mammary lesions. The inhibitory concentration 50 of bistriazole was calculated in cell cultures of patients with benign lesions, Probit = 4.6 μM with IC = 95%. The study was performed by examining 63 women who submitted to mammary biopsies. Biopsies of the mammary lesions were performed, gene expression was determined, and cells were cultured in the presence of 4.6 μM bistriazole. We found that breast cancer is related to age greater than 50 (P ≤ 0.01), being overweight (P ≤ 0.023) and having a waist circumference larger than 80 cm (P ≤ 0.01). The gene expression of JAK2, STAT3, and NF-κB was higher in groups of patients with breast cancer, while SOCS3 expression was lower. After being exposed to bistriazole, the expression of JAK2 and STAT3 decreased, and the expression of SOCS3 and NF-κB increased. In conclusion, this molecule in development has an effect on the gene expression of JAK3 and STAT3; nevertheless, the lack of change in NF-κB indicates that it is not a regulator of inflammation, and therefore, more studies should be performed.
Agid:
4593568