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Aronia melanocarpa fruit extract exhibits anti-inflammatory activity in human aortic endothelial cells
- Zapolska-Downar, D., Bryk, D., Małecki, M., Hajdukiewicz, K., Sitkiewicz, D.
- European journal of nutrition 2012 v.51 no.5 pp. 563-572
- Aronia melanocarpa, adhesion, anti-inflammatory activity, antioxidant activity, atherosclerosis, cardioprotective effect, cell adhesion, endothelial cells, flow cytometry, fruit extracts, fruits, humans, leukocytes, messenger RNA, phosphorylation, polyphenols, protein synthesis, surface proteins
- BACKGROUND: Altered expression of cell adhesion molecules (CAMs) has been implicated in a variety of chronic inflammatory conditions, including atherosclerosis. Regulation of adhesion molecule expression by specific redox-sensitive mechanisms has been reported. Additionally, it has been observed that the extract of Aronia melanocarpa (A. Melanocarpa) fruits, rich in polyphenols, exhibits potent anti-oxidant properties and displays cardioprotective activity. METHODS AND RESULTS: Human aortic endothelial cells (HAECs) were pretreated with various concentrations (primarily 50 μg/mL) of Aronia Melanocarpa fruit extract prior to treatment with TNFα (10 ng/mL) for various periods of time. The surface protein and mRNA expression of ICAM-1 and VCAM-1 were determined using flow cytometry and real-time RT-PCR, respectively. Adhesion of peripheral blood mononuclear leucocytes (PBMLs) to TNFα-treated HAECs was evaluated by an adhesion assay. Activation of NF-κB was evaluated by measuring NF-κB p65 phosphorylation using flow cytometry. ROS production was determined by reduction in fluorescent 2′,7′-dichlorofluorescein diacetate (DCFH-DA). Tested A. Melanocarpa extract significantly inhibited the expression of ICAM-1 and VCAM-1, attenuated the phosphorylation of NF-κB p65 and decreased intracellular ROS production in TNFα-treated HAECs. CONCLUSION: We conclude that A. Melanocarpa fruit extract exhibits anti-inflammatory effects in HAECs by inhibiting the expression of endothelial CAMs, activation of NF-κB and production of ROS.