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Fine mapping of a yield-enhancing QTL cluster associated with transgressive variation in an Oryza sativa x O. rufipogon cross

Xie, Xiaobo, Jin, Fengxue, Song, Mi-Hee, Suh, Jung-Pil, Hwang, Hung-Goo, Kim, Yeon-Gyu, McCouch, Susan R., Ahn, Sang-Nag
Theoretical and applied genetics 2008 v.116 no.5 pp. 613-622
DNA, Oryza rufipogon, Oryza sativa, alleles, chromosomes, cultivars, heading, inflorescences, introgression, isogenic lines, phenotype, quantitative trait loci, rice
A high-resolution physical map targeting a cluster of yield-related QTLs on the long arm of rice chromosome 9 has been constructed across a 37.4 kb region containing seven predicted genes. Using a series of BC₃F₄ nearly isogenic lines (NILs) derived from a cross between the Korean japonica cultivar Hwaseongbyeo and Oryza rufipogon (IRGC 105491), a total of seven QTLs for 1,000-grain weight, spikelets per panicle, grains per panicle, panicle length, spikelet density, heading date and plant height were identified in the cluster (P <= 0.0001). All seven QTLs were additive, and alleles from the low-yielding O. rufipogon parent were beneficial in the Hwaseongbyeo background. Yield trials with BC₃F₄ NILs showed that lines containing a homozygous O. rufipogon introgression in the target region out-yielded sibling NILs containing Hwaseongbyeo DNA by 14.2-17.7%, and out-yielded the Hwaseongbyeo parent by 16.2-23.7%. While higher yielding plants containing the O. rufipogon introgression were also taller and later than controls, the fact that all seven of the QTLs were co-localized in the same 37.4 kb interval suggests the possibility that a single, pleiotropic gene acting as a major regulator of plant development may control this suite of agronomically important plant phenotypes.