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Effect of barley β-glucan on murine RAW264.7 macrophages against virulent Salmonella enterica serovar Typhimurium

Fan, Yang-Guo, Hu, Chia-Wen, Chu, Chishih, Chiu, Kuan-Chi, Weng, Brian Bor-Chun
Food research international 2012 v.45 no.2 pp. 1106-1110
Salmonella enterica subsp. enterica serovar Typhimurium, antibacterial properties, barley, blood, cholesterol, diarrhea, gene expression, hosts, humans, immune response, lysozyme, macrophages, mice, pathogens, reactive oxygen species, receptors, rehydration, soups, therapeutics, zoonoses
Heavily evidenced in antimicrobial effects of microbial β-(1,3)(1,6)-glucan, the botanical β-(1,3)(1,4)-glucan is mostly shown to effectively control blood cholesterol level. S. Typhimurium belongs to food-borne zoonoses often causing worldwide epidemic outbreaks in animals and human with severe diarrhea and gastrointestinalitis. Cereal soups are commonly prescribed as neutraceutical for rehydration purpose and a sustaining therapy. Botanical β-(1,3)(1,4)-glucan is easily released in boiled cereal soup. β-(1,3)(1,4)-Glucans on modulating host defense to enteric infectious agents is seldom reported. Our results demonstrated that barley β-(1,3)(1,4)-glucans effectively increased the murine macrophage cell line RAW264.7 against S. Typhimurium infection through antibacterial lysozyme activity (P<0.001), not the production of intracellular reactive oxygen species. Furthermore, barley β-(1,3)(1,4)-glucans upregulated the gene expressions of its receptor dectin-1. In conclusion, barley β-(1,3)(1,4)-glucan induces a mild immune response with increasing antibacterial lysozymes through up-regulating its receptors dectin-1 and lysozyme M gene expressions.