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Effect of barley β-glucan on murine RAW264.7 macrophages against virulent Salmonella enterica serovar Typhimurium
- Fan, Yang-Guo, Hu, Chia-Wen, Chu, Chishih, Chiu, Kuan-Chi, Weng, Brian Bor-Chun
- Food research international 2012 v.45 no.2 pp. 1106-1110
- Salmonella enterica subsp. enterica serovar Typhimurium, antibacterial properties, barley, blood, cholesterol, diarrhea, gene expression, hosts, humans, immune response, lysozyme, macrophages, mice, pathogens, reactive oxygen species, receptors, rehydration, soups, therapeutics, zoonoses
- Heavily evidenced in antimicrobial effects of microbial β-(1,3)(1,6)-glucan, the botanical β-(1,3)(1,4)-glucan is mostly shown to effectively control blood cholesterol level. S. Typhimurium belongs to food-borne zoonoses often causing worldwide epidemic outbreaks in animals and human with severe diarrhea and gastrointestinalitis. Cereal soups are commonly prescribed as neutraceutical for rehydration purpose and a sustaining therapy. Botanical β-(1,3)(1,4)-glucan is easily released in boiled cereal soup. β-(1,3)(1,4)-Glucans on modulating host defense to enteric infectious agents is seldom reported. Our results demonstrated that barley β-(1,3)(1,4)-glucans effectively increased the murine macrophage cell line RAW264.7 against S. Typhimurium infection through antibacterial lysozyme activity (P<0.001), not the production of intracellular reactive oxygen species. Furthermore, barley β-(1,3)(1,4)-glucans upregulated the gene expressions of its receptor dectin-1. In conclusion, barley β-(1,3)(1,4)-glucan induces a mild immune response with increasing antibacterial lysozymes through up-regulating its receptors dectin-1 and lysozyme M gene expressions.