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The protective effect of schisandra lignans on stress-evoked hepatic metastases of P815 tumor cells in restraint mice

Tang, Shu-Hong, He, Rong-Rong, Huang, Ting, Wang, Cong-Zhi, Cao, Yun-Feng, Zhang, Yang, Kurihara, Hiroshi
Journal of ethnopharmacology 2011 v.134 no.1 pp. 141-146
adverse effects, cytotoxic T-lymphocytes, high performance liquid chromatography, lignans, metastasis, mice, neoplasms, oral administration, protective effect, splenocytes
AIM OF THE STUDY: The present study was conducted to investigate the effects of schisandra lignans extract (SLE) on stress-evoked hepatic metastases of mastocytoma P815 tumor cells, which was closely related with immune function. MATERIALS AND METHODS: The high-performance liquid chromatography (HPLC) fingerprint of SLE was recorded and the percentage composition of schisandra lignans was determined as 82.63%. The contributions of the immunomodulatory properties of SLE to the protective effects on stress-induced hepatic metastases were studied. RESULTS: Our results found that restraint stress significantly promoted hepatic metastases of P815 tumor cells. However, oral administration of SLE (100 and 200mg/kg/d, 14d) significantly reduced the number of metastatic colonies in liver of restrained mice. SLE was further found to be significantly improving T lymphocyte proportions and increasing cytotoxic T lymphocyte (CTL) activity of immunized spleen cells in stressed mice. CONCLUSION: These results indicated that the protective effects of SLE on hepatic metastases were related to its alleviation of the adverse effects of stressors for bio-homeostasis and immunoprotection. The obtained data provided evidences to elucidate the traditional use of Fructus schisandrae as a tonic or sedative.